Stromal Cell-Derived Factor-1 alpha (SDF-1 alpha) Promotes Growth and Migration of Bone Marrow Stromal Cells (BMSCs) and Gastric Cancer Cells Through Phosphatidylinositol 3-Kinase/AKT (PI3K/Akt) Pathway

SDF-1 alpha activity is closely related to information transmission and cell migration when contributing to lymphatic metastasis in various tumors. Herein, we explored the interaction among SDF-1 alpha, CXCR4 and PI3K/Akt signaling pathway in gastric cancer (GC) and their roles in this disorder. Human GC cells KATO-III and BMSCs were co-cultured without contact. GC cells were transfected with SDF-1 alpha, CXCR4 inhibitor, and PI3K inhibitor. After examining the efficiency of transfection, cell migration was evaluated using Transwell chamber, and expression SDF-1 alpha, CD133, and CXCR4 was determined by RT-qPCR. With transfection rate of 98%, the number of migrated cells reduced upon inhibition of CXCR4 and PI3K. Luciferase activity in 565 nm are high than CXCR4 inhibition group. (p < 0.05). Likewise, up-regulation of SDF-1 alpha increased the expression of SDF-1 (0.825 +/- 0.061), CD133 (0.875 +/- 0.058), CXCR4 (0.801 +/- 0.052), and Akt (0.852 +/- 0.062), compared to the blank group, CXCR4 inhibition group and PI3K inhibition group (p 0.05). Down-regulation of CXCR4 and PI3K, however, decreased the expression insignificantly (p 0.05). Collectively, up-regulation of SDF-1 alpha IP: 49.249.253.194 On: Thu, 18 Nov 2021 07:32:12 Copyright: American Scientific Publishers activates CXCR4 signaling pathway of BMSCs and stimulaes its downstream PI3K/Akt signaling Delivered by Ingenta pathway and and increases the expression of CD133, thereby promoting malignant behaviors of GC cells.