MOLECULAR CLONING OF AMYLASE ENCODING GENE FROM BACILLUS SUBTILIS

The study aimed to amplify the amylase coding gene from B. Subtilis & Clone the gene in E.Coli. The amylase-producing microorganisms were isolated using starch agar media. Isolation of genomic DNA from the Characterized strain. Then the 5'-CCTACAACATTTGTGC-3' and TAATCTCGAGTACGTATTTAAAAGTCACGTAC3' primers were used to amplify the DNA using the following cycle in PCR Method, The amplification reaction of 40 cycles was generated & bands with sizes ranging from 675 bps & up to 9742 bps. DNA fragment of 2000bps carrying the amylase gene was obtained & it was cloned in PUC 18 plasmid. E.Coli was used as a host to clone the gene.As a result.from the soil sample,the present study deals with the cloning and expression of amylase gene from Bacillus subtilis to E.coli was expressed to produce more amount of the industrial important amylase enzyme.