Mowing increased plant diversity but not soil microbial biomass under N-enriched environment in a temperate grassland

Aims The anthropogenic nitrogen (N) input has considerable consequences on soil microbial biomass, which is critical for biogeochemical cycling. As prevalent grassland management, mowing may reduce soil N storage, enhance plant biodiversity and soil microbial carbon (C) availability, all of which are important regulators of soil microbial biomass. However, convincing data is still scarce about how mowing affects soil microbial biomass under N enrichment. Methods The interactive effects of N addition (0 – 50 g N m −2 yr −1 ) and mowing (unmown vs. mown) on soil microbial biomass C (MBC) were measured by manipulating 6 years’ N addition experiment in Inner Mongolia grassland of China. Results Mowing increased soil inorganic N concentration, available Cu 2+ concentration, plant aboveground net primary production (ANPP), species richness, Shannon-Wiener biodiversity and ratio of fungal to bacterial biomass, and decreased soil available Mn 2+ concentration under N enrichment. Mowing also significantly reduced the dominance of Leymus chinensis . While mowing did not affect the soil MBC compared with that in only N added plots. The soil MBC was positively regulated by plant species richness and biodiversity, while was negatively regulated by ANPP, soil inorganic N, available Cu 2+ and Mn 2+ concentration in mown plots. Conclusions The results highlight that mowing cannot mitigate the negative effects of N enrichment on soil MBC. The soil, plant and microbial properties play important roles in regulating the response of soil MBC to mowing in N-enriched soil. This work improves the mechanistic understanding of the linkages between plant community and soil microbial C cycling.