Study on the influence of incubation temperature on specific protein detection results based on COMSOL simulation

Incubation temperature plays an important influential role in the detection process of specific proteins. In this paper, a specific protein detection device was designed and built by taking Lambert-Beer's law as the basic detection principle. By using the photoelectric effect of the photoelectric sensor, the relative absorbance of the transmissibility sample was detected by the turbidimetric method, and then the substance content in the detected sample was quantitatively analyzed. Meanwhile, the increment PID algorithm was used to control the temperature in the incubation area, and then the finite element analysis method and COMSOL finite element simulation software were employed to analyze the temperature distribution and temperature variation trend in the incubation area of the reaction device. Besides, the microalbumin kit of Beijing Dandan Biotechnology was used to detect the influence of incubation temperature on the calibration curve of microalbumin (mALB) and its changing trend under different temperatures. The experimental results indicated that the increasing PID control algorithm could control the average temperature rise rate of the incubation area at 0.52 degrees C/s and the temperature stability at +/- 0.1 degrees C. When the incubation temperature was between 34 degrees C and 40 degrees C, the change of temperature had no significant effect on the slope of the calibration curve. Within this temperature range, the linearity of the calibration curve gradually increased with the increase of the temperature. This study provided a basic experimental study on the effect of incubation temperature on the detection results of specific proteins.