A ternary biocomposite based on modified fish scale collagen and ginsenoside Rb1: preparation, properties and bioactivities

This paper presents some characteristics, morphologies and bioactivities of modified collagen films (MCF) in a combination of fish scale collagen gel, crosslinking agent and ginsenoside Rb1. Collagen was extracted from freshwater fish scales by biochemical methods using pepsin enzyme to improve collagen hydrolysis. Glutaraldehyde, glucose and riboflavin were chosen as modification agents for the obtained collagen. These collagen samples were processed into MCF by a 3D printing method. The characteristics and morphologies of MCF were evaluated using infrared, energy-dispersive X-ray, field emission scanning electron microscopy and high-performance liquid chromatography techniques. Moreover, the biocompatibility, hemostatic ability and anti-inflammatory and antimicrobial activities of MCF were tested for preliminarily assessing the application efficiency of MCF. The obtained results suggest that crosslinking agent and ginsenoside Rb1 have effects on properties of collagen. MCF are well biocompatible, have great anti-inflammatory and antimicrobial ability, are nontoxic to normal cells and have short blood clotting time. They are promising for applications of 3D printed modified collagen/ginsenoside Rb1 biocomposite films in biomedicine as hemostatic agents or other biomedical materials. (c) 2022 Society of Industrial Chemistry.