AbsorbanceQ: An App for Generating Absorbance Images from Brightfield Images

The AbsorbanceQ app converts brightfield microscope images into absorbance images that can be analyzed and compared across different operators, microscopes, and time. Because absorbance-based measurements are comparable across these parameters, they are useful when the aim is to manufacture biotherapeutics with consistent quality. AbsorbanceQ will be of value to those who want to capture quantitative absorbance images of cells. The AbsorbanceQ app has two modes - a single image processing mode and a batch processing mode for multiple images. Instructions for using the app are given on the 'App Information' tab when the app is opened. The input and output images for the app have been defined, and synthetic images were used to validate that the output images are correct. This article provides a description of how to use the app, software specifications, a description of how the app works, instructive advice on how to use the tools and a description of the methods used to generate the software. In addition, links are provided to a website where the app and test images are deployed.