Organizing principles of the prefrontal cortex from a single-neuron projectome

An efficient tool for neurite tracing has been developed that reconstructed the complete axons of 6,357 individual projection neurons in the mouse prefrontal cortex (PFC). The resulting single-neuron projectome analysis revealed comprehensive PFC neuron subtypes, topographic organization of PFC axon projections, modular structure within the PFC and correspondence with single-cell transcriptomes.