Paenibacillus farraposensis sp. nov., isolated from a root nodule of Arachis villosa

Strain UY79(T) was isolated from a root nodule of Arachis villosa, collected at the Esteros de Farrapos National Park. Rio Negro, Uruguay. Cells were non-motile Gram-variable rods with central to subterminal oval to ellipsoidal endospores that swell the sporangia. Growth was observed in the range of 15-42 degrees C (optimum, 30 degrees C), pH 5.0-9.0 (optimum. pH 7.0-8.0) and with up to 3% (w/v) NaCl (optimum. 1-2%). Strain UY79(T) was facultative anaerobic. catalase-positive and oxidase-negative. According to the results of 16S rRNA, gene sequence analysis, UY79(T) belongs to the genus Paenibacillus and is closely related to P. ottowii MS2379(T), P. peoriae BD-57(T), P polymyxa ATCC 842(T) and P brasilensis PB172(T). exhibiting 99.4, 99.0, 99.0 and 98.9% sequence identity, respectively. Average nucleotide identity and digital DNA-DNA hybridization values with the most closely related type strains were 74.3-88.6% and 38.2-48.7%, respectively. Major fatty acids (>10%) were anteiso-C-15:0, iso-C-15(:0), and C-16:0. Menaquinones MK-7 and MK-6 were the only isoprenoid quinones detected. Major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unidentified glycolipid. Spermidine was the predominant polyamine. The DNA G+C content based on the draft genome sequence was 46.34mol%. Based on the current polyphasic study, UY79(T) represents a novel species of the genus Paenibacillus, for which the name Paenibacillus farraposensis sp. nov. is proposed. The type strain is UY79(T) (=CCM 9147(T) =CGMCC 1.19038(T)).