Isolating Venomous Constituents of Paralepistopsis acromelalga and Simultaneous Analysis of Its Compounds

An isolation method for Acromelic acids A, B and Clitidine, which are venomous constituents of Paralepistopsis acromelalga was developed. Highly purified products were obtained from the mushroom extract using silica gel, ODS, ion-exchange column chromatography and preparative TLC. Using those results, we optimized the LC-MS/MS conditions. Finally we developed a method for simultaneous analysis. In recovery tests, the average recovery was 80.8-112.4%, repeatability was 1.4-3.8RSD %. The limits of quantification of the respective compounds were estimated as 0.25 mu g/g. Based on the results, this method can reveal causes of food poisoning by Paralepistopsis acromelalga.