Aliidiomarina halalkaliphila sp. nov., a haloalkaliphilic bacterium isolated from a soda lake in Inner Mongolia Autonomous Region, China

A haloalkaliphilic strain (IM 1326(T)) was isolated from brine sampled at a soda lake in the Inner Mongolia Autonomous Region, China. Cells of the strain were rod-shaped and motile. Strain IM 1326(T) was able to grow at 4-42 degrees C (optimum, 37 degrees C) with 0-13.0% (w/v) NaCl concentrations (optimum at 4.0-6.0%) and at pH 7.5-11.0 (optimum at 9.0-10.0). The 16S rRNA gene phylogenetic analysis revealed that the isolate belongs to the genus Aliidiomarina and is closely related to the type strains of Aliidiomarina sanyensis (95.8% sequence similarity), Aliidiomarina shirensis (95.7%), Aliidiomarina iranensis (95.4%) and Aliidiomarina haloalkalitolerans (95.3%). The whole genome of strain IM 1326(T) was sequenced, and the genomic DNA G+C content was 49.7mol%. Average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values between the isolate and the related Aliidiomarina species were 68.1-84.9%, 76-78% and 18.4-20.4%, respectively. The respiratory quinone was ubiquinone-8. The polar lipid profile included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and one unidentified aminophospholipid. The predominant cellular fatty acids were summed feature 9 (10-methyl-C-1(6.)0/iso-C-17:1 omega 9c. 22.2%), iso-C-1(5:)0 (16.1%) and iso-C-17(:0) (13.1%). Based on the results of phylogenetic analysis, genome relatedness, and the physiological and chemotaxonomic properties of the isolate, strain IM 1326(T) is considered to represent a novel species of the genus Aliidiomarina, for which the name Aliidiomarina halalkaliphila sp. nov. is proposed (type strain IM 1326(T)=CGMCC 1.17056(T)=JCM 34227(T)).