Leclercia pneumoniae sp. nov., a bacterium isolated from clinical specimen in Leipzig, Germany

Strain 49125(T) was isolated from an infant with pneumonia and septicaemia at the Leipzig University Hospital. Phenotypic and genomic traits were investigated. The strain's biochemical profile and its MALDI-TOF spectrogram did not differ from comparative samples of Leclercia adecarboxylata, thus far the sole member of the Leclercia species. A circular genome with a size of 4.4 Mbp and a G+C content of 55.0 mol% was reconstructed using hybrid Illumina and Nanopore sequencing. Phylogenetic analysis was based on 172 marker genes and validated using a k-mer-based search against a large genome collection including subsequent in silico DNA-DNA hybridization. Whole genome average nucleotide identity to any described species was below 95%, suggesting that strain 49125 1 represents a new species, for which we propose the name Leclercia pneumoniae sp. nov. with the type strain 49125(T) (=LMG 32245(T) =DSM 112336(T)).