Antifungal Mechanism of Fubaiju Essential Oil According to Labeling Method

Fubaiju essential oil was prepared by organic solvent extraction and steam distillation method. The cells,cell membrane,nucleic acid,mitochondrion and circular plasmid pCAMBIA1303 of S. cerevisiae were labeled with Jianna green, 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate, propidium iodide,rhodamine 123 and ethidium bromide respectively to investigate antifungal mechanism of Fubaijuessential oil. According to the results of fluorescence microscope, Fubaiju essential oil showed antifungal activity against S. cerevisiae. Fubaiju essential oil could not only destroy the cell membrane, make the cell contents leak out, but also penetrate the cell membrane, destroy the mitochondrial membrane and DNA,and eventually lead to cell death.