Molecular characterization of Indian Dolichos bean (Lablab purpureus L. var. typicus Prain) accessions using RAPD markers

In this study, 29 genotypes of (Lablab purpureus) Indian bean augmented from different geographical regions were analyzed for genetic diversity using 39 RAPDs. The binary matrix data revealed 97.4% polymorphism among genotypes, with 2322 amplification fragments. The PIC values ranged from 0.25 to 0.96 with an average of 0.70. Markers OPA05, OPB15, and OPV12 showed high discrimination among all the genotypes. A magnificent range of Jaccard's genetic similarity coefficient was evaluated and UPGMA based dendrogram classified genotypes into 4 major clusters. IC-384066 and NSJ/NAIP/192 were found to be unique and highly diversified. The same grouping patterns were also observed in the model-based STRUCTURE analysis (K=2). The study showed the degree and distribution of genetic diversity in Dolichos bean, which can be utilized to identify the parental lines, to develop mapping populations and breeding.