Nano-Exopolysaccharide From The Probiotic Weissella Paramesenteroides MN2C2: Production, Characterization And Anticancer Activity

Exopolysaccharides nanoparticles (EPS-NPs), especially from probiotics, became one of the most innovative approaches for intelligent cancer therapy due to their stability and biocompatibility. In a previous study performed by the research team, a polysaccharide, produced by Weissella paramesenteroides MN2C2 was identified as a sulphated hetero-polysacchraide. In this study, this exopolysaccharide was partially purified and the cultivation period, pH and temperature were optimized. Nano-forms of crude and partially purified EPS were synthesised by sonication and characterized by Transmission Electron Microscopy (TEM) and Dynamic Light Scattering (DLS). The particles were platelet-like and their size diameter ranged from 37 to 105 and from 45 to 204 nm for crude and partially purified EPS-NPs respectively. Both EPS-NPs were effective and highly selective against Colon Caco-2, Liver HepG-2 and Breast MCF-7 malignant cells. They were also found to have potent DPPH antioxidant activities dependent on their concentration. The producing probiotic strain showed high sensitivity towards Tetracycline, Fusidic acid and Ampiciline with no haemolytic activity. And it was tolerant to low pH, bile salts and pancreatic juices, and exerted antimicrobial activity against Pseudomonas aeruginosa and Bacillus subtilis. These findings suggest that nano-EPS from the probiotic W. paramesenteroides possess excellent characteristics for potential medicine applications.