A new caffeic acid ester and a new ceramide from the roots of Eriosema glomeratum

A new caffeic acid ester, eriocaffeate (1), and a new ceramide, erioceramide (2) along with eight known compounds, genistein (3), genistin (4), lupinisoflavone A (5), quercetin (6), lupeol (7), a mixture of beta-sitosterol (8) and stigmasterol (9), and beta-sitosterol-3-O-beta-D-glucopyranoside (10) were obtained after the phytochemical investigation of the CH2Cl2/MeOH (1:1, v/v) extract from the roots of Eriosema glomeratum. The structures of the new compounds were elucidated by analyses of their spectroscopic data including 1D- and 2D-NMR, HRESI-MS, and ESI-MS/MS as well as chemical conversions. The cytotoxicity of isolated compounds 1 6 was determined against three human [lung (A549), breast (MCF7), and cervical (HeLa)] cancer cell lines. Compounds 3 and 6 possessed the activities on all the tested three cell lines, MCF7 (IC50 = 88.9 and 42.6 mu M), A549 (IC50 = 42.1 and 28.2 mu M), and HeLa (IC50 = 27.4 and 19.1 mu M), respectively. Compound 4 showed selective moderate activity with the IC50 value of 68.7 mu M on the HeLa cancer cell line.