Computational Approaches for RNA Structure Ensemble Deconvolution from Structure Probing Data

•RNA molecules populate a dynamic ensemble of alternative structures.•RNA structure probing data provide an ensemble-averaged snapshot of structural signatures.•Ensemble deconvolution methods can be classified as thermodynamics-dependent or independent.•The first class uses thermodynamics to identify a set of folds most consistent with the data.•The second class directly clusters sequencing reads into structural subpopulations.