Assessing gene function in human B cells: CRISPR/Cas9-based gene editing and mRNA-based gene expression in healthy and tumor cells

Federica Nardi, Laura Pezzella, Roberta Drago,Anthea Di Rita,Martina Simoncelli,Giuseppe Marotta,Alessandro Gozzetti,Monica Bocchia,Anna Kabanova

EUROPEAN JOURNAL OF IMMUNOLOGY（2022）

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摘要

Robust methods for manipulation of human B cells, isolated from healthy donors and patients with B cell disorders, has the potential to significantly accelerate B cell research. Our work describes a step-by-step protocol to perform electroporation-based screening of gene function in B cells through the use of Cas9 ribonuclecomplexes and in vitro produced mRNA.

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关键词

B cells, Chronic lymphocytic leukemia (CLL), CRISPR, Cas9, mRNA, Screening

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