A duplex droplet digital PCR assay for Salmonella and Shigella and its application in diarrheal and non-diarrheal samples

Jingyun Zhang,Yuanming Huang,Panpan Xue,Zhifei Zhan,Zheng Huang,Jie Li,Baowei Diao,Biao Kan

International Journal of Infectious Diseases（2022）

引用 4|浏览10

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摘要

•The duplex ddPCR assay targets Salmonella fimY and Shigella ipaH genes.•LOD and LOQ of ddPCR were 550 and 5,500 CFU/mL of stool sample for Shigella.•Both LOD and LOQ were 1.0 × 104 CFU/mL of stool sample for Salmonella.•ddPCR gave more positive results than qPCR in detecting stool samples.•Salmonella load was higher in diarrheal samples than in non-diarrheal samples.

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关键词

Salmonella,Shigella,Droplet digital PCR,Real-time PCR,Children with diarrhea,Children without diarrhea

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