Dynamics of transition dipole orientation in representative fluorescent proteins

Molecules of fluorescent proteins behave like antennas: they exhibit distinct directionality of light absorption and emission. In our previous work we determined the mean orientations of the ‘molecular antennas’ (transition dipole moments) within the atomic framework of fluorescent protein molecules. In the presented work we use molecular dynamics simulations, as well as time resolved fluorescence anisotropy measurements, in order to investigate the dynamics of transition dipole moment orientation in representative fluorescent proteins.