Analysis of Deamidation and Oxidation in Monoclonal Antibody using Peptide Mapping with UPLC/MSE

This application note demonstrates that UPLC-MSE is a suitable tool for characterizing PTMs in monoclonal antibodies. Using UPLC-MSE with the ACQUITY UPLC and SYNAPT MS systems, the identification of peptide sequences and determination of site-specific moditications can be accomplished in a single LC run because of available peptide fragmentation information provided by MSE.