Protocol for PPL Solid Phase Extraction for Dissolved Organic Matter (DOM) Sample Preparation for LC-MS/MS Analysis v1

Authors: Monica Thukral, Irina Koester, Daniel Petras, Ralph Riley Torres, Allegra Aron, Emily Gentry, Xavier Siwe-Noundou, Rosemary Dorrington, Kerry McPhail, Aaron Hartmann, Lihini Aluwihare General Remarks: Protocol for PPL Solid Phase Extraction (SPE) for sample preparation for LC-MS/MS analysis of DOM. Using a SPE vacuum station, this protocol can be adapted for large (>200 mL) or smaller sample volumes (<200 mL). The general procedure is written for larger volumes with notes embedded with procedural modifications for smaller samples. Examples of recommendations for sample size are 1 L for environmental samples, and 50-100 mL for a laboratory culture. Avoid materials with silicone coating and PEG contaminants (e.g. silicon tubing and sterivex and supor filters).