Holotomographic imaging of eukaryotic cells

Abstract Holotomography measures 3D refractive index (RI) distribution in cells and tissues without exogenous labeling. Here we describe a protocol for holotomographic imaging of generic eukaryotic cells using a standardized Tomocube holotomographic microscope. Combined with the recent advances in machine learning, holotomographic imaging enables a broad range of new biological and medical applications.