Wastewater QC workflow in GalaxyTrakr (SSQuAWK) v2

Jasmine Amirzadegan, Tunc.kayikcioglu Not Provided, Hugh.rand Not Provided,Ruth E Timme, Maria Balkey

semanticscholar(2022)

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摘要
PURPOSE: Step-by-step instructions for checking sequence quality for SARS-CoV-2 wastewater samples using SSQuAWK: SARS - CoV - 2 Sequence Quality Assurance Workflow and Kontraption. The SSQuAWK workflow, implemented in a custom Galaxy instance, will produce quality assessments for raw reads (Illumina MiSeq paired-end fastq files). SCOPE: This protocol covers the following tasks: 1. Set up an account in GalaxyTrakr 2. Create a new history 3. Upload data 4. Execute the SSQuAWK workflow 5. Interpret the results Version history: V1: Basic protocol steps with screenshots V2: Addition of a detailed 12 minute video tutorial
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