Multiplexed bio-assay for a reliable detection of HER2 in human plasma using a combined label-free/fluorescence biosensing platform

Human epidermal growth factor receptor 2 (HER2) over-expression occurs in 15–20% of breast cancers and it is generally associated with a dismal prognosis. In this work, we report on the use of one-dimensional photonic crystal biochips to detect clinically relevant concentrations of HER2 in human plasma samples. To this aim, we optimized an optical read-out system, combining both label-free and fluorescence detection, which makes use of biochips tailored with specific proteins for specific biological recognition. Our biochips were used to discriminate HER2 positive/negative human plasma samples providing a solid and reliable tool for clinical diagnostics.