Colorimetric detection of SARS-CoV-2 by uracil-DNA glycosylase (UDG) reverse transcription loop-mediated isothermal amplification (RT-LAMP)

Meng Yee Lai,Fatma Diyana Mohd Bukhari,Nur Zulaikha Zulkefli,Ilyiana Ismail,Nur Izati Mustapa,Tuan Suhaila Tuan Soh,Afifah Haji Hassan,Kalaiarasu M Peariasamy,Yee Leng Lee,Jeyanthi Suppiah,Ravindran Thayan,Yee Ling Lau

International Journal of Infectious Diseases（2022）

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摘要

•RNA-purification–free method was developed for the detection of SARS-CoV-2•One-step uracil-DNA glycosylase reverse transcription loop-mediated isothermal amplification was used to detect SARS-CoV-2•Result were obtained by direct observation of end products by the naked eye

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关键词

RT-LAMP,UDG,dUTP,Carryover contamination,SARS-CoV-2

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