Differential and organ-specific functions of organic solute transporter α and β in experimental cholestasis

•This manuscript describes the first mouse model of OSTβ deficiency.•Ostβ-/- mice are viable and fertile, but show increased length and weight of the small intestine, blunted villi and deeper crypts.•Ostβ deficiency leads to an altered microbiome compared to both wild-type and Ostα-/- mice.•Cholestasis led to lower survival and worse body weight loss, but an improved liver phenotype, in Ostβ-/- mice compared to Ostα-/- mice.