Silver Nanoparticle-Loaded Gelatin-Based Nanocomposite Films toward Enhanced Mechanical Properties and Antibacterial Activity

Gelatin (GH) is a natural polymer material with unique physical, chemical, and biological properties that render it a good base material for biomedical material production. Herein, Ag nanoparticles (NPs) were loaded onto a waterborne polyurethane-GH composite (WPU-g-GH) to prepare a GH-based nanocomposite (AgNP/WPU-g-GH) films). The prepared nanocomposite films were characterized using several analyses including Fourier transform infrared spectroscopy, ultraviolet-visible spectroscopy, X-ray diffraction, transmission emission microscopy, mechanical strength tests, and other analyses. The results demonstrated that the nanocomposite films had high mechanical strength, good thermal stability, and controllable biodegradability. In particular, when the AgNP loading content was 0.03%, the tensile strength, elongation at break, and average particle size of the nanocomposite film reached 45.13 MPa, 476.04%, and 13.02978 +/- 1.64406 nm, respectively. Disk diffusion and cytotoxicity analyses revealed that the nanocomposite films exhibited significant antibacterial activity against Gram-negative and Gram-positive bacteria without affecting the cell viability of fibroblasts. These findings indicate that the nanocomposite films with high mechanical strength and antibacterial activity could be used for wound management, tissue adhesion, and biomaterial surface coating.