Automated stain-free histomorphometry of peripheral nerve by contrast-enhancing techniques and artificial intelligence
Journal of Neuroscience Methods(2022)
摘要
•Fast myelin histomorphometry using DIC and phase contrast microscopy is demonstrated.•Deep learning is used to predict myelin fluorescent images from label-free images.•Machine learning is employed for automated myelinated axon counts.•Machine learning is employed for semi-automated g-ratio measurements.
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关键词
Fluorescence,Microscopy,Histology,Nerve fibers,Peripheral nerve
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