Integration of Nucleic Acid Amplification, Detection, and Melting Curve Analysis for Rapid Genotyping of Antimicrobial Resistance

To prevent the overuse of antibiotics and reduce the spread of antimicrobial resistance (AMR), we developed an integrated genotypic test to identify target AMR genes and mutations with minimal user operation. The integrated AMR sensor consists of a reaction chamber built on a total internal reflection (TIR)-coupled DNA microarray, a temperature management unit, and a compact fluorescence reader. B...