Tetrahedral structure supported two stages DSN-assisted amplification strategy for sensitive detection of lung cancer related MicroRNA

•We propose here a novel florescence assay for accurate analysis of miRNA via duplex specific nuclease (DSN) signal amplification.•A favorable detection sensitivity through the DSN enzyme based signal amplification;•Florescence signal generation in a label-free way based on MG/G-quadruplex complex;•A stable signal generation even in complicated experimental conditions based on the tetrahedral structure.