Distinct subpopulations of DN1 thymocytes exhibit preferential gamma delta T lineage potential

Seungyoul Oh,Xin Liu,Sara Tomei, Mengxiao Luo, Jarrod P. Skinner, Stuart P. Berzins,Shalin H. Naik,Daniel H. D. Gray,Mark M. W. Chong

FRONTIERS IN IMMUNOLOGY(2023)

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摘要
The alpha beta and gamma delta T cell lineages both differentiate in the thymus from common uncommitted progenitors. The earliest stage of T cell development is known as CD4(-)CD8(-) double negative 1 (DN1), which has previously been shown to be a heterogenous mixture of cells. Of these, only the CD117(+) fraction has been proposed to be true T cell progenitors that progress to the DN2 and DN3 thymocyte stages, at which point the development of the alpha beta and gamma delta T cell lineages diverge. However, recently, it has been shown that at least some gamma delta T cells may be derived from a subset of CD117(-) DN thymocytes. Along with other ambiguities, this suggests that T cell development may not be as straightforward as previously thought. To better understand early T cell development, particularly the heterogeneity of DN1 thymocytes, we performed a single cell RNA sequence (scRNAseq) of mouse DN and gamma delta thymocytes and show that the various DN stages indeed comprise a transcriptionally diverse subpopulations of cells. We also show that multiple subpopulations of DN1 thymocytes exhibit preferential development towards the gamma delta lineage. Furthermore, specific gamma delta-primed DN1 subpopulations preferentially develop into IL-17 or IFN gamma-producing gamma delta T cells. We show that DN1 subpopulations that only give rise to IL-17-producing gamma delta T cells already express many of the transcription factors associated with type 17 immune cell responses, while the DN1 subpopulations that can give rise to IFN gamma-producing gamma delta T cell already express transcription factors associated with type 1 immune cell responses.
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关键词
T cell development,gamma delta (γδ) T cells,lineage decision,scRNAseq,thymocyte
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