Biological Response of Irisin Induced by Different Types of Exercise in Obese Subjects: A Non-Inferiority Controlled Randomized Study

Simple Summary Among healthy male and female obese individuals undergoing a 12-week aerobic exercise program with either moderate intensity endurance or high-intensity interval training for losing weight, a reduction of circulating irisin was observed. Irisin is an important adipo-myokine implicated in the regulation of energy metabolism and cardiovascular health. Sex differences in the circulating levels of this biomarker have been previously reported and are likely related to the different anthropometric features between the sexes. A sex-specific modulation of circulating irisin levels should be further explored to tailor sex-specific training approaches for improving the cardiovascular health of obese subjects. Background: Weight loss through physical exercise is warranted among obese individuals. Recently, a greater benefit in cardiorespiratory fitness was achievable with high-intensity interval training (HIIT) as compared with moderate intensity continuous training. The beneficial effect of training on CV health might be related to a specific modulation of circulating irisin, an adypo-myokine implicated in the regulation of energy expenditure. Methods: The present study investigates the circulating plasma levels of irisin at baseline and in response to 12-week of training program either with HIIT or moderate-intensity continuous training (MICT) among young female and male obese subjects. Clinical, anthropometric, and training characteristics for each participant were available. A sex-disaggregated data for circulating plasma levels of irisin pre- and post-training are provided as well as an adjusted multivariate linear regression model to identify the determinants of post-training irisin levels. Results: Data from a total of 32 obese healthy individuals (47% female, mean age 38.7 years, mean BMI 35.6 kg/m(2)), randomized in a 1:1 manner to HIIT or MICT were analyzed. Circulating plasma levels of irisin similarly and significantly decreased in both MICT and HIIT interventional groups. Females had higher post-exercise irisin levels than males (6.32 [5.51-6.75] vs. 4.97 [4.57-5.72] mu g/mL, p = 0.001). When stratified by an interventional group, a statistically significant difference was observed only for the MICT group (male, 4.76 [4.20-5.45] mu g/mL vs. female 6.48 [4.88-6.84] mu g/mL p = 0.03). The circulating post-training level of irisin was independently associated with post-training fat-free mass (beta -0.34, 95% confidence interval, CI -0.062, -0.006, p = 0.019) in a model adjusted confounders. When female sex was added into the adjusted model, it was retained as the only factor independently associated with irisin levels (beta 1.22, 95% CI, 0.50, 1.93, p = 0.002). Conclusions: In obese healthy subjects, circulating irisin levels were reduced in response to 12-weeks of exercise involving either HIIT or MICT. A sex-specific differences in circulating irisin levels at baseline and as biological response to chronic exercise was described. Sex-specific biological response of irisin to exercise should be further explored to tailor sex-specific training approaches for improving the cardiovascular health of obese healthy subjects.