Phytochemical screening and chemical compound of green roselle(Hibiscus sabdariffa L.) and potential antibacterial activities

Abstract Herbal rosella plants (Hibiscus sabdariffa L.) are plants that have great potential in the pharmaceutical field. Indonesian Sweetener and Fiber Crops Research Institute (ISFCRI) has released four superior herbal rosella varieties, including Roselindo-1, Roselindo-2, Roselindo-3, and Roselindo-4. Of the four varieties, Roselindo 3 has different physical characteristics, namely green. The purpose of this study was to determine the chemical and phytochemical compounds contained in the green rosella extract. Green rosella extract was obtained using the Microwave-Assisted Extraction (MAE) method with ethyl acetate as a solvent. The results of phytochemical screening for green roselle extract contained flavonoids, tannins, and triterpenoids. Based on the results of Liquid Chromatograph Mass Spectrometry (LCMS) analysis, green roselle extract contains 323 compounds, including 5-hydroxymethyl-2-furaldehyde, 7-hydroxycoumarine, betaine, curcumin, nicotinamide, and jasmonic acid.