A New Lipid-Droplets-Targeted Fluorescence Probe with Dual-Reactive Sites for Specific Detection of ClO- in Living Cells

Lipid droplets (LDs) are organelles composed of a lipid core surrounded by a phospholipid monolayer, plays an important role in a variety of physiological processes as well as diseases. In this work, TPAs-SCH3-2CN with an enhanced electron D-pi-A system, is synthesized, which makes itself as an orange emission compound (E-m=590 nm) and also have high polarity. It is verified that TPAs-SCH3-2CN is an excellent LDs-targeted probe, and it also sensitive to the ClO- detection in vitro and in cells. Impressively, due to the high lipophilicity, TPAs-SCH3-2CN is able to stain the yolk lipids in zebrafish, exhibiting the potential for monitoring lipid transport and metabolism processes.