Neuroprotective and Therapeutic Effects of Tocovid and Twendee-X on A beta Oligomer-Induced Damage in the SH-SY5Y Cell Line

Background: Alzheimer's disease (AD) is the most frequent cause of dementia among the elderly. The accumulation of amyloid beta (A beta) and its downstream pathological events such as oxidative stress play central roles in AD. Recent studies revealed that A beta oligomer (A beta O)-induced strong neurotoxicity in SH-SY5Y cells via the induction of oxidative stress. Objective: In the present study, we investigated the effect of two antioxidants, Tocovid and Twendee-X, on A beta O-induced SH-SY5Y cell damage. Methods: A beta Os (2.5 mu M) were applied to induce cellular damage in the SH-SY5Y cell line. Cell viability following A beta O toxicity, Tau protein phosphorylation, cell morphology, and intracellular reactive oxygen species were assayed with or without different concentrations of Tocovid or Twendee-X. Results: Tocovid (60 mu g/mL) and Twendee-X (150 mu g/mL) significantly recovered cell viability from A beta O toxicity (**p < 0.01, vs. control), attenuated Tau protein phosphorylation (**p < 0.01, vs. A beta Os), improved cell morphology (**p < 0.01, vs. A beta Os), and suppressed intracellular ROS (**p < 0.01, vs. A beta Os) in SH-SY5Y cells. Conclusion: These findings suggest the neuroprotective and therapeutic potential of Tocovid and Twendee-X for AD treatment. (C) 2022 S. Kager AG, Basel.