Isolation from Streptomyces of a Novel Naphthoquinone Compound, Naphthablin, that Inhibits Abl Oncogene Functions.

In the course of our screening for inhibitors of abl oncogene function, a culture nitrate of Streptomyces aculeolatus. induced normal flat morphology in v-abl-expressing NIH3T3 cells. The active substance was isolated through ethyl acetate extraction, silica gel column chromatography, and reverse-phase HPLC. Mass and NMR spectroscopy including HMBC revealed that it had a novel naphthoquinone structure with a monoterpene, and we named it naphthablin. Naphthablin inhibited Abl-induced morphological transformation in v-ablts-NIH3T3 cells at around 30μg/ml, and specifically inhibited RNA synthesis.