Identification of An Inactive y-Type High-Molecular-Weight Glutenin Gene in Crithopsis delileana (Schult.) Roshev.

Using SDS-PAGE analysis, the high-molecular-weight glutenin subunits (HMW-GS) of two Crithopsis delileana (Schult.) Roshev. accessions were detected. It was found that the two accessions had the same HMW-GS. Only one HMW-GS with the similar electrophoresis mobility to the y-type HMW-GS of hexaploid wheat was observed in C. delileana. It was not sure whether the y-type HMW glutenin genes were silenced or not. By using other protein extraction methods and sequencing of partial gene coding region, it was confirmed that, in fact, the y-type HMW glutenin gene in C. delileana accessions was silenced.