Sequence analysis of the entire genes of an avian influenza virus A/TurKey/England/N28/73(H5N2)

Reverse Transcription-Polymerase Chain Reaction (RT-PCR)was used to amplify the eight full-length cDNAs of an Avian Influenza Virus(AIV) A/TurKey/England/N28/73(H5N2).The cDNAs were then cloned to PMD18-T vector and sequenced.The results showed that each of eight gene cDNAs fragments contains the whole open reading frame of each gene.TEN28/73 was characteristic of Low Pathogenic Avian Influenza Viruses.Phylogenetic analysis of HA gene showed that TEN28/73 was not closely related to A/Goose/Guangdong/1/96和A/Goose/Guangdong/3/97;phylogenetic analysis of NA gene showed that TEN28/73 was most closely related to A/Duck/Hongkong/86/76.