Reseach of the mechanism of severe acute pancreatitis and the therapeutic effect of Panaxadiol Saponin

Objective To investigate the role of TNF-αand COX-2 in the development of severe acute pancreatitis (SAP) and the therapeutic effect of Panaxadiol Sapenin(PDS).Methods 120 SD rats were randomly divided into sham operation group,SAP model group, PDS treated group and Dexamethasone treated group.There were 30 rats in each group.Rats in each group were killed 6,12 and 24 h (10 rats for each time point) after operation to determine the serum levels of TNF-α.The pancreas tissues of the rats of 6 h after operation were obtained to conduct HE dye and immunohistochemistry assay to examine the changes with microscope and the expression of COX-2.Results The pancreas tissues in sham operation group had no obvious changes.It could be seen in model group that pancreas tissue necrosis,blood vessel breach and bleeding and inflammation cell infiltration.The changes in PDS and Dexamethasone treated group were less than those of model group.Serum TNF-αlevels in model group were higher than that in sham operation group(P0.01) and PDS and Dexamethasone treated group (P0.05).The expression of COX-2 in model group,PDS and Dexamethasone treated group was 90%,60% and 50% re- spectively.The expression of COX-2 in model group were higher than that of PDS and Dexamethasone treated group(P0.05).Conclu- sions TNF-αinduces the developing of SAP and increases the expression of COX-2,PDS can reduce the inflammation response of SAP.