Subchronic oral toxicity of genetically modified cottonseed with FBP7-iaaM gene in rats

Objective To study the subchronic oral toxic effects of genetically modified(GM) cottonseed with FBP7-iaaM gene in rats.Methods The rats were fed with diets containing GM cottonseed with FBP7-iaaM gene or containing non-GM cottonseed at a dose of 5%,2.5%,1.25% and 0%(control group) respectively for three consecutive months,the general behavior,body weight,food consumption,food efficiency,etc,were observed.At the mid and end of experiment,the blood and serum samples were collected for routine and biochemical assays;the internal organs were also taken for calculating the viscera coefficients and doing histopathological examinations.Results The rats exhibited free movements and shining coats without any abnormal symptoms and abnormal secretions in their noses,eyes or mouths.There were no adverse effects on body weight in GM cottonseed groups and non-GM cottonseed groups,no biological differences in hematological and biochemical indices were observed;no significant differences(P0.05) in viscera coefficient were detected between experimental groups and control group.No histopathological changes were observed.Conclusion In the present study,no adverse effects of GM cottonseed with FBP7-iaaM gene and its parent strain control were seen in rats.