Effect of vitamin C on inhibition of proliferation and apoptosis of human embryo fibroblasts

Objective To explore the proliferation and apoptosis of human embryo fibroblast-1(HFL-Ⅰ)cell lines after cultured with vitamin C.Methods HFL-Ⅰ cell lines were cultured with 0,40,400,4 000 μg/ml vitamin C,respectively.The cell viability was measured by CCK-8 assay.Flow cytometry was used to analyze the cell cycle.Collagen expression was measured by Sirius red staining.The expression of Caspase-3 mRNA was detected by reverse transcription-polymerase chain reaction(RT-PCR).Results Vitamin C inhibited the growth of HFL-Ⅰ cell lines in a dose-dependent manner(P0.05),and reduced the formation of collagen(P0.05).Flow cytometry results showed that the cells were delayed in the G1 phase.Vitamin C significantly up-regulated the mRNA of Caspase-3 after cultured with vitamin C for 12 h.Conclusion Vitamin C can inhibit the proliferation of HFL-Ⅰ,reduce the formation of collagen,block HFL-Ⅰ cells in G1 phase,and induce the apoptosis of HFL-Ⅰ cells by up-regulating the expression of Caspase-3 mRNA.