Rapid detection of drug-resistant genes of clinical isolates in Mycobatterium tuberculosis by Multi-analyte Suspension Array

104 Mycobacterium tuberculosis strains and H_(37)Rv strain as control were genotyped by mutiPCR based on Luminex 100~(?) platform for detecting the mutant sequences in rpoB,katG and inhA gene to identify the result of the drug resistance.Of 104 strains,Mutations were found in 73 strains(98.6%) of 74 randomly selected rifampin-resistant MTB,50 strains(86.2%) of 58 randomly selected isoniazidresistant MTB and 48 strains(90.6%) of 53 randomly selected both rifampin and isoniazid-resistant MTB by MASA.Multi-analyte Suspension Array technology has high sensitivity and specificity in detection of drug-resistant of MTB and may be applied in clinical diagnosis.