Construction of luciferase expression vector containing C-MET and characterization of the promoter activity

【Objectives】 To make a luciferase reporter gene construct with promoter region-223~+60 from human C-MET gene,and to determine its activity in the ovarian cancer cell lines.【Methods】 The-223~+60 region of C-MET promoter was amplified by polymerase chain reaction(PCR) and recombinated into pGL3-Basic vector.The recombinated plasmid pGL3-C-MET-Promoter was confirmed by restriction analysis and sequencing.Transcient transfected pGL3-C-MET-Promoter into four ovarian cancer cell lines and detected its activity.【Results】 The results showed that the sequence of cloned promoter was right,the promoter construct has higher activity in OVCAR3 cells compared to others.【Conclusion】 The luciferase reporter gene vector containing C-MET promoter has been successfully constructed and it establishes the foundation to future research on the influence to the biology behavious of ovarian cancer.