Phân lập và xác định đặc tính sinh học của một số chủng porcine circovirus type 2 (PCV2) từ heo nuôi tại khu vực phía nam Việt Nam

This study aimed to isolate and examine the biological char-acteristics of PCV2 field strains circulating in Vietnam for further study in producing vaccine against PMWS (postweaning multisystemic wasting syndrome). Eighteen PCV2 strains were successfully isolated and belonged to genotype PCV2b (9 strains), PCV2d (6 strains) and recombinant (3 strains). The viral titers of PCV2 isolates at the third passages in PK15A cells were in the range from 1.67 to 5,50 log10TCID50/mL. Three PCV2 field strains with stable viral titers (≥5,0 log10TCID50/mL) through passages, which belonged to different genotypes, were selected as master seed for studying of PCV2 vaccines.