Best practices in cell culture: an overview Animal

John M. Baust, Gertrude C. Buehring,Lia Campbell, Elmore E,John W. Harbell,Raymond W. Nims, Paul J. Price,Yvonne Reid, Frank P. Simione

In Vitro Cellular & Developmental Biology – Plant(2017)

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摘要
This overview describes a series of articles to provide an unmet need for information on best practices in animal cell culture. The target audience primarily consists of entry-level scientists with minimal experience in cell culture. It also include scientists, journalists, and educators with some experience in cell culture, but in need of a refresher in best practices. The articles will be published in this journal over a six-month period and will emphasize best practices in: (1) media selection; (2) use and evaluation of animal serum as a component of cell culture medium; (3) receipt of new cells into the laboratory; (4) naming cell lines; (5) authenticating cell line identity; (6) detecting and mitigating risk of cell culture contamination; (7) cryopreservation and thawing of cells; and (8) storing and shipping viable cells.
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