Research on the key technology of nucleic acid detection device

With the rapid development of nucleic acid molecule detection technology, nucleic acid detection devices have become a global research hotspot in recent years. This paper uses multidisciplinary fusion technology to propose a nucleic acid fluorescence detection design scheme. The device is mainly composed of a motion module and an optical system. Among them, the motion module uses an S-type variable acceleration control method to precisely control the movement of the motor. The stable and accurate operation lays the foundation for the collection of fluorescent signals; the optical system uses LED as the fluorescent excitation light source. The LED collimated uniform light path is designed. Use image stitching to stitch the collected images into a complete image for analysis. Finally, measure the operating error of the motor, the average error does not exceed 14um; simulate the fluorescence signal acquisition system and collect the image of the fluorescent film, the image fluorescence is uniform, which verifies the rationality of the fluorescence acquisition system; collects simulated samples and blank control images , Analyze the stitched images, and the results clearly distinguish the simulated samples and the control group.