Microbiological studies on diarrhoeic sheep in Southern Rajasthan

The present study was undertaken to find out the bacteriological etiology in 64 sheep affected with diarrhea in southern region of Rajasthan. Bacteria isolated were E. coli in 36.92%, Pseudomonas 6.15%, Klebsiella 9.23%, Yersinia 7.69%, and Citrobacter 9.23% Pasteurella 7.69% and No bacterial growth in 21.87% of the sample collected. E. coli serotype identified were O135 in 25 %, O26 in 12.5% and untypable in 62.5%. Molecular confirmation of E coli and pseudomonas isolates was done by amplification of uspA & Oprl gene by using PCR. Antibiogram patterns of different bacterial organisms isolated from various sample of diarrheic sheep showed varying degree of sensitivity to the chemotherapeutic agents. Antibiogram patterns observed for various bacteria will be useful in prevention and treatment of diarrhaea.