Dynamic study of three-dimensional peroxynitrite flux in epileptic brain using high-speed and high-resolution photoacoustic imaging

In this study, we proposed the linear array ultrasound transducer based photoacoustic imaging (PAI) method for peroxynitrite flux study when having Epilepsy. To our best knowledge, this is the first study using high-speed and high-resolution imaging system that can achieve study of the dynamic flux of peroxynitrite in the whole brain. A novel PAI probe was designed based on high frequency linear array ultrasound transducer for high-resolution brain imaging. Sparse sampling based compressed sensing combined with arc-rotary scanning strategy was proposed to achieve high speed PAI of the whole Brain. A mice epileptic model was established, which was continuously monitored for the dynamic flux of peroxynitrite within the brain using a peroxynitrite sensitive photoacoustic probe: Manganese (II) Texaphyrin (MMn). The dynamic neurochemical mechanisms in the process of epileptic seizure was investigated and the correlation between epilepsy burst and severe neuron injury was explored.