Bioprinting 3D angiogenesis models with Laser Induced Side Transfer

The ability to generate 3D angiogenesis models is central for tissue engineering and drug screening applications. However, existing bio-fabrication technologies have yet to attain precise guidance of capillary networks in 3D. Here, we present our latest results in fabricating capillary networks using a novel laser-assisted bioprinting technique named Laser Induced Side Transfer (LIST). We found that LIST-printed human umbilical vein endothelial cells (HUVECs) present negligible loss of viability and maintain their abilities to migrate, proliferate and form intercellular junctions. Furthermore, we showed that LIST enabled the formation of capillary-like networks in 3D with high spatial precision (50 μm) over a large volume (1 cm3). Those networks were validated as angiogenesis assays for pro- and anti-angiogenic compounds. LIST could be widely adapted for applications requiring multiscale bioprinting capabilities, like the development of 3D drug screening models and artificial tissues.