Fluorescence imaging through dynamic scattering media with and without using wavefront shaping

Adaptive Optics and Wavefront Control for Biological Systems VII(2021)

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摘要
Fluorescence imaging is indispensable to biomedical research, and yet it remains challenging to image through dynamic scattering samples. Wavefront shaping has enabled fluorescence imaging through scattering media. However, the translation of these techniques into in vivo applications has been hindered by the lack of high speed solutions to counter the fast speckle decorrelation of dynamic tissue. Here, we report an ultrasound enabled optical imaging method that instead leverages the dynamic nature to perform imaging. The method utilizes the correlation between dynamic speckle encoded fluorescence and ultrasound modulated light signal that originate from the same location within a sample. We successfully imaged fluorescent targets within a dynamic scattering medium.
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